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Tie'n hanh so sanh vdi cac trinh tuf gien Japonica ma sd: NM Ddng thdi sQ dung chuong thanh xu the mdi trong cac nghien cQu thie't ke trinh nay chuimg toi cung da xay dung dugc cay cac vector dugc nhidu phdng thi nghiem su dung quan be phat sinh cua gien MtOsDREB2A vdi cac do cd nhieu uu didm: 1.
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Vi vay ra't tien lgi cho cac Thiet ke vector chuyen gien theo ky thuat phdng thf nghiem cin thie't ke nhieu vector gateway: He thd'ng gateway dua tren phan Qng chuydn gien. Vector nhan hinh 3 cd bd khung la vector Ubiquitine, vi trf MCS va trat tu ket thuc phien pBIlOl, gan them vao mdt cassette gdm ma NosT dugc gidi ban hai dau la vi trf cit ciia promoter, gien ccdB va gien chloramfenicol va enzim ta' td hgp clonase attLl, attL2. Gien ccdB la gien cua vuus, rit ddc cho cho tha'y vdi 11 khuin lac sinh trudng tren mdi E.
Cac khuan lac sinh EHA sQ dung phuong pblp xung didn. Su trudng tren mdi trudng LB cd bd sung tdn tai cua vector chuyen gien trong vi khuin kanamyein dugc kiem tra su tdn tai cua casstte Agrobacterium dugc khang dinh thdng qua phan mang gien cin chuyen thdng qua phan Qng PCR iing PCR vdi cac khuin lac sinh trudng tren mdi vdi mdi xudi GW-F bam dac hieu phfa cud'i trudng LB cd bd sung kanamyein vdi cap mdi vung NosT ben trii va mdi dac hieu dac bieu cho gien MtOsDREB2A.
Neu phan Qng clonase thanh hinh 3B cho tha'y vdi 10 khuan lac chung tdi thu cdng cassette mang gien cin chuydn dugc dugc 3 khuan lac so 1, 9 va 10 cho ke't qua chuydn sang vector nhan thi phan Qng PCR se duong tinh vdi san phim khuech dai rd net cd tao ra va san phim khue'ch dai cd kfch thudc kfch thudc xa'p xi bp. Khuan lac sd 10 dugc theo tinh toan khoang 2,9 kb. Kfch thudc 2,9 kb lua chgn de lam nguyen lieu cho tbi nghiem tuong Qng vdi su cdng ddn kfch thudc gien chuydn gien thuc vat. Ke't qua Callus lua tren moi trudng MS bo sung 2,4D; B.
Callus liia tren mdi trudng ddng nuoi ciy; C. Callus liia tren moi trudng chon loc. Tai sinh callus liia; E. Liia chuyen gien sau 3 thang; F. TQ hat lua gid'ng chung tdi cd the md rdng va tien hanh ddng thdi Chanh trui dugc vao nudi ca'y, chQng tdi thu cac thf nghiem chuyen gien khac nhau vao cay dugc 91 callus sau 10 ngay nudi cly tren mdi lua. Sau 15 ngay A. CIc cay chuydn gien sau dd cd kha mdi trudng chQa hygromycin.
Nhu vay, hieu nang sinh trudng binh thudng trong didu kien suit chuydn gien cua gid'ng lua Chanh trui la nha kfnh cung nhu tren rudng thf nghiem. Sau 4 tuln nudi ca'y tren mdi trudng tai Loi cdm on: Cdng trinh dugc tai trg kinh phf sinh, cac cay lua non phat tridn td't dugc chuydn bdi Chuong trinh Cdng ngh6 sinh hgc Ndng sang mdi trudng ra rd hinh 4A, B, C va D.
Sau 10 ngay, cay dugc chuyen sang cac cdc dat va nghiep - Cao Le Quyen va cs. Bartels D.
Kfch thudc theo tfnh toan cua san 3. Dubouzet J. Ke't qua dien 4.http://host.successintheworld.com/15874-tracking-cheating-boyfriend.php
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Liu Q. Nishimura A. Cac cay nay sau 6. Qin F. Sakuma Y. Seki M. Shen Y. Day Umezawa T. Xue G. Ky thuat gateway Zhang J. As a result, from Chanh trui seeds for callus formation, 25 lines transgenic rice plants were identified and PCR analysis demonstrates that 16 lines transgenic rice plants have contained the interested gene in genome. Ngdy nhdn bdi: Related Papers. Development of transgenic duckweed expressing VP2 antigen and the preliminary immunological in test chickens.
By Tien Vu Van. By Nhon Dang Hoai.
10 dấu hiệu cảnh báo
A: Yes. Last year , the Peoria announced they would consider re-voting on the issue if the University leadership consented to a return. So far, the Board of Trustees and other University leaders have not revisited the issue. Q: What can I do to support the Chief tradition?
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A: The Honor the Chief Society is the leading organization in preserving the Chief tradition open to community members and alumni alike. Current students can also join Students for Chief Illiniwek, a registered student organization on campus. Q: What do I say to someone who criticizes me for supporting the Chief? A: Encourage them to learn more about the issue.
The greatest threat to the Chief tradition is that people neither fully know the history of the Chief, nor do they understand the meaning and culture for which it stands. A: Just as you have the freedom to speak out that you are offended, so too should we be allowed the freedom to express our support. Like fixing a car rather than buying a new one, those in support of the Chief tradition strive to make it as respectful as possible rather than abandoning it entirely. Q: If the Chief tradition is inclusive to all students, why has every Chief portrayer been a white frat boy?
A: That is not the case. Past portrayers and assistants include men and women and draw from those both within and outside of the Greek system. Portrayers have claimed multiple ethnicities from Native to Hispanic. Q: Does the Chief regalia represent traditional Illini clothing? Regalia is modeled after more of a Plains tribe style than what would have been seen in Illinois. He saw this as a way to honor the positive way in which the Chief was portrayed.
The current regalia is modeled after that regalia, which has been returned to South Dakota. Although the dance may have been based upon traditional moves at its inception, each of the 37 subsequent Chief portrayers have made minor alterations to make the dance their own. Because of this, the dance now is almost totally different from when it began in Q: How can you defend the Chief when both the dance and attire lack authenticity?
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A: The difference is largely in the intent. Even modern Native gatherings often feature competitive dancing, featuring dances that have been modified for entertainment purposes and colorful clothing that would not have been traditionally available. These dances serve to catch the attention of the general public, as well as a younger generation of Native children so that they become interested in the culture that these modern performances were based on.
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These pow-wow dances and the Chief dance alike serve as ways to preserve culture, share it with a new audience, and stimulate interest for further study. Q: Research done at Illinois shows that the Chief symbol elicits a feeling of aggression and conflict.
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How can this be true if the Chief is not offensive? A: This feeling comes from the fact that students are aware of and have experienced the controversy surrounding the symbol and has nothing to do with the symbol itself. In fact, the National Congress of American Indians uses a similar logo. Q: Stereotyping and offensive imagery are more prevalent than they have ever been on campus. Is the Chief to blame? A: Quite the opposite, actually.